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. 2016 Jan 7;98(1):185–201. doi: 10.1016/j.ajhg.2015.12.002

Figure 5.

Figure 5

CFTR Regulatory Elements Are Contained within One TAD

(A–E) Zoomed-in view of the 5C chromatin interaction map. TAD5 is highlighted by the black bar. Insulation profiles for each heatmap section are displayed below each heatmap.

(F and L) Genome browser snapshot of TAD5, showing the two genes ASZ1 and CFTR located in the TAD.

(G–K) 3C-style chromatin interaction profile anchored on the ASZ1 promoter (probe REV_404). This gene is not expressed in any of the five cell lines.

(M–Q) 3C-style chromatin-interaction profile anchored on the CFTR promoter (REV_421). This gene is expressed in Caco2, Calu3, and Capan1 cell lines. For (G–K) and (M–Q), the orange bar represents the location of the anchoring interaction. The dotted black lines represent the Loess standard deviation, and the thick black line represents the Loess average. The red line represents the interactions of the genomic fragment indicated by the orange line with all other interrogated fragments in the region. Significant interactions are indicated by large circles. Grey lines and genomic coordinates indicate locations (kb from the promoter) of known CFTR enhancer elements.