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Mean S/P ratio of ELISA result after in ovo immunization with pIRES-HN/F or pIRES-HN/F-D-SPM complexes.
Notes: The error bars show standard deviation of means. S/P ratio = Sample mean (mean of optical absorbance) – negative control mean/positive control mean – negative control mean.
Abbreviations: ELISA, enzyme-linked immunosorbent assay; pIRES, plasmid internal ribosome entry site; HN, hemagglutinin; F, fusion; D-SPM, dextran-spermine; Nano, nanoparticle.
