Table 1.

PCR primer sequence, annealing temperature, and product size, as well as restriction enzyme used and band sizes obtained after digestion, for genotyping of the polymorphisms

SNP	Primer sequence	Annealing temperature (°C)	PCR product size (bp)	Restriction enzyme	Genotype (bp)	References
rs2243250	5′-TAAACTTGGGAGAACATGGT-3′	52	195	Avall	TT: 195	15
	5′-TGGGGAAAGATAGAGTAATA-3′				CT: 195+176+19
					CC: 176+19
rs10499563	5′-AGGGAAAGCAGGTTATCAAA-3′	61	424	Taal	TT: 424	16
	5′-AGTGGCTTCAGGGAGACTAA-3′				TC: 424+320+104
					CC: 320+104
rs4073	5′-ATCTTGTTCTAACACCTGCCACTCT-3′	59	121	Munl	TT: 121	17
	5′-TAAAATACTGAAGCTCCACAATTTGG-3′				TA: 121+82+39
					AA: 82+39
rs2233406	5′-GGTCCTTAAGGTCCAATCG-3′	62	200	Bfal	CC: 180+20	18
	5′-GTTGTGGATACCTTGCACTA-3′				CT: 200+180+20
					TT: 200
rs3138053	5′-GGTCCTTAAGGTCCAATCG-3′	62	200	TspRl	AA: 200	18
	5′-GTTGTGGATACCTTGCACTA-3′				AG: 200+129+71
					GG: 129+71

Abbreviations: PCR, polymerase chain reaction; SNP, single-nucleotide polymorphism.