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. 2016 Jan 14;9:325–333. doi: 10.2147/OTT.S95985

Figure 4.

Figure 4

Cell cycle and cell apoptosis of SMMC-7721 cells that were transfected with TMEM16A siRNA.

Notes: (A) Cell cycle distribution was measured by flow cytometry with cell cycle staining kit and (B) the cell cycle phase is shown in a bar graph with the G0/G1, S, and G2/M phases. (C) Cell apoptosis in each group was determined using the Annexin V-FITC/PI flow cytometry, and (D) proportion of apoptosis cells was measured. Control: normal SMMC-7721 cells; NC: negative control; siRNA: cells transfected with TMEM16A siRNA. *P<0.05 vs control.

Abbreviations: FITC, fluorescein isothiocyanate; PI, propidium iodide; V-FITC/PI, fluorescein isothiocyanate-conjugated Annexin V and PI.