Table 2.

Results of primer screening for 13 polymorphic loci developed for Fosterella christophii.^a

Locus	NW09.005 (N = 11)			NW09.030 (N = 9)			NW09.034 (N = 9)			F. christophii (N = 29)	F. micrantha (N = 31)	F. villosula (N = 21)	Total (N = 81)
	A	Ho	He	A	Ho	He	A	Ho	He	A	A	A	Atotal
Foc_01	4	0.09*	0.59	5	0.44	0.72	4	0.78	0.78	7	9	4	13
Foc_02	2	0.00	0.42	5	0.78	0.75	5	1.00	0.83	8	4	6	13
Foc_03	3	0.09	0.50	4	0.56	0.60	4	0.22	0.47	9	8	2	16
Foc_04	1	—	—	2	0.22	0.52	6	0.89	0.80	9	1	1	9
Foc_05	2	0.00*	0.52	5	0.78	0.71	3	0.11	0.57	7	2	2	9
Foc_06	1	—	—	4	0.89	0.66	3	0.56	0.57	6	4	2	8
Foc_07	2	0.00	0.42	2	0.22	0.21	4	0.67	0.75	4	2	2	4
Foc_12	2	0.00*	0.52	4	0.56	0.61	4	0.67	0.70	7	11	3	15
Foc_18	2	0.00	0.17	5	0.44	0.66	4	0.00*	0.65	7	4	2	11
Foc_25	2	0.09	0.25	1	—	—	3	0.89	0.69	4	3	2	6
Foc_27	3	0.09	0.18	4	0.78	0.71	4	0.67	0.75	7	5	4	8
Foc_28	1	—	—	3	0.56	0.60	2	0.33	0.29	4	4	2	6
Foc_30	2	0.00	0.51	2	0.67	0.52	4	0.22	0.78	6	4	5	9
Mean	2.08	0.04	0.41	3.54	0.57	0.61	3.85	0.54	0.66	6.54	4.69	2.85	9.77

Note: A = number of alleles; A_total = number of alleles across all tested accessions; H_e = expected heterozygosity; H_o = observed heterozygosity; N = number of individuals sampled.

^aSee Appendix 1 for locality and voucher information.

*Highly significant deviation from Hardy–Weinberg equilibrium (P ≤ 0.001).