Table 1.
Primer sequences for the eight putative single-copy nuclear markers developed to amplify across Melastomataceae.
| Locus | Primer sequences (5′–3′) | Putative Arabidopsis homolog | Putative product | Ta (°C) |
| ADH | F: TGGCAAGCACYGCTGGTCAGG | At1g77120 | Alcohol dehydrogenase | 56 |
| R: GAGATGCCGCAGCTSAGGA | ||||
| UNC50 | F: CGGGAGGAGGCACCAAATAG | At2g15240 | UNC-50 family protein | 53 |
| R: AGARGCGGCCACCATGAAGA | ||||
| PCRF1 | F: RCTCAAGTTCGAGAGTGGAGT | At2g47020 | Peptide chain release factor 1 | 50 |
| R: YAGCTTTGACCGATCCRAGT | ||||
| UFP | F: AAGTGGAGCGGGAAGGAGTA | At4g06599 | Ubiquitin family protein | 53 |
| R: RGGAGMCTCCACTTGGTCCA | ||||
| At5g10780 | F: AGYGCCTTGCAGGGTGTTGG | At5g10780 | Unknown protein | 50 |
| R: RAGTAGGCCCAATGTGTTTA | ||||
| SOS4 | F: GCAACAAGTCGGCTGTCTTT | At5g37850 | Pyridoxal kinase | 51 |
| R: CGGAGCTTCTTGACAACTTCC | ||||
| ZBADH | F: TCCACGAGCTYGGGGGTRCC | At5g61510 | Zinc-binding alcohol dehydrogenase family | 53 |
| R: GGCCGGAAGAATCTGCTCTT | ||||
| waxy | F1: GRGGTCTTGGGGACGTGCTC | At1g32900 | Granule-bound starch synthase 1 | 54 |
| F2: ACACTTGCGTGGTCGTYCAG | ||||
| R: AGCAGTGTGCCARTCGTTGG |
Note: Ta = annealing temperature used in the PCR.