Appendix 1.

List of genera across the Melastomataceae in which amplification of the eight putative single-copy nuclear markers was successful.

Clade	Species	ADH	At5g10780	PCRF1	SOS4	UFP	UNC50	waxy	ZBADH	Voucher (Herbarium)a
Bertolonieae	Bertolonia mosenii Cogn.	+	+	+	+	+	+	+	+	Goldenberg, R. 804 (NY, UPCB)
Blakeeae	Blakea bracteata Gleason	+	+	+	+	+	+	+	+	Goldenberg, R. 964 (NY, UPCB)
Melastomeae	Brachyotum quinquenerve (Ruiz & Pav.) Triana	+	+	+	+	+	+	+	+	Michelangeli, F. A. 1979 (NY)
Marcetia alliance	Comolia microphylla Benth.	+	+	+	+	+	+	+	+	Michelangeli, F. A. 2499 (NY)
Henrietteeae	Henriettea trachyphylla (Triana) Penneys, Michelang., Judd & Almeda	+	+	+	+	+	+	+	+	Almeda, F. 10165 (CAS)
Cambessedesia alliance	Huberia consimilis Baumgratz	+	+	+	+	+	+	+	+	Michelangeli, F. A. 1618 (NY)
Merianieae	Meriania nobilis Triana	+	+	+	+	+	+	+	+	Clark, J. L. 13051 (UNA)
Olisbeoideae	Mouriri chamissoana Cogn.	—	—	+	+	+	+	+	—	Reginato, M. 1025 (UPCB)

Note: + = successful amplification; — = no amplification.

^aVouchers are deposited at the herbaria of the California Academy of Sciences (CAS), San Francisco, California, USA; New York Botanical Garden (NY), Bronx, New York, USA; University of Alabama (UNA), Tuscaloosa, Alabama, USA; or Universidade Federal do Paraná (UPCB), Curitiba, Paraná, Brazil.