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. 2015 Dec 23;37(2):387–397. doi: 10.3892/ijmm.2015.2444

Figure 2.

Figure 2

Transduction of transactivator of transcription (Tat)-superoxide dismutase (SOD) proteins into human umbilical vein endothelial cells (HUVECs). Tat-SOD or control SOD (0.1–3 µM) was added to HUVECs for 2 h (A). Tat-SOD or control SOD (3 µM) was added to HUVECs for 10–120 min (B). After HUVECs were treated with 3 µM Tat-SOD for 2 h, Tat-SOD expression in HUVECs for 1–60 h was examined by western blot analysis (C). Data indicates the means ± SEM; n=5 experiments. Columns without a common letter differ, P<0.05. HUVECs were treated with various doses of Tat-SOD or SOD for 2 h. To identify cellular translocalization of Tat-SOD, cells were transduced with 1–3 µM FITC-stained Tat-SOD or SOD for 2 h. Cellular localization of Tat-SOD protein was observed by counterstaining with DAPI, using fluorescence microscopy (D). C, control.