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. 2015 Dec 23;37(2):387–397. doi: 10.3892/ijmm.2015.2444

Figure 5.

Figure 5

Inhibition of cellular induction of occludin-1, platelet/endothelial cell adhesion molecule 1 (PECAM-1) and vascular endothelial (VE)-cadherin and monocyte transmigration by transactivator of transcription (Tat)-superoxide dismutase (SOD). Human umbilical vein endothelial cells (HUVECs) were treated with 10 ng/ml tumor necrosis factor-α (TNF-α) for 24 h in the presence and absence of Tat-SOD or control SOD. To measure the cellular expression of occludin-1, PECAM-1 and VE-cadherin, total cell lysates were subjected to western blot analysis with a primary antibody against occludin-1, PECAM-1 and VE-cadherin (A). β-actin was used as an internal control. The bar graph in the right panel represents quantitative results of blots obtained by densitometric analysis. To examine the transmigration of TNF-α-treated THP-1 monocytes, THP-1 cells were loaded onto Transwell inserts and exposed to 10 ng/ml TNF-α for 24 h in the presence of Tat-SOD (B and C). Transmigrated monocytes were stained with toluidine blue and the staining intensity was quantified, and microphotograph images were taken with a light microscope (3 separate experiments). Magnification, x200. The columns represent the means ± SEM, and variables without a common letter differ, P<0.05; n=3 experiments.