Skip to main content
. 2015 Dec 11;37(2):347–358. doi: 10.3892/ijmm.2015.2433

Figure 1.

Figure 1

Nitric oxide synthase 2 (NOS2) expression and nitric oxide (NO) production induced by interferon-γ (IFN-γ) and/or interleukin-17 (IL-17) in macrophages. (A-a, B and C-a) Representative western blot analysis of NOS2 in RAW 264.7 cells treated with indicated cytokines for 24 h. (A-b and C-b) Average quantification obtained by densitometric analysis for western blot analysis. Data are expressed as the density ratio of target protein to its non-treated level in arbitrary units. (D) Expression of NOS2 at the mRNA level in RAW 264.7 cells treated with indicated cytokines for 12 h. β-actin was selected as a housekeeping gene. (E) Nitrite concentration in the supernatant of cultured RAW 264.7 cells treated with indicated cytokines for 24 or 48 h. (F-a) Representative western blot analysis and (F-b) quantification of NOS2 in peritoneal macrophages treated with indicated cytokines for 24 h. β-actin was used as a loading control. Data are presented as the means ± SD from three independent experiments. *p<0.05 and **p<0.01.