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. 2015 Dec 11;37(2):347–358. doi: 10.3892/ijmm.2015.2433

Figure 2.

Figure 2

Signal transducer and activator of transcription 1 (STAT1) pathway and nitric oxide synthase 2 (NOS2) expression induced by interferon-γ (IFN-γ) and/or interleukin-17 (IL-17) in RAW 264.7 cells. (A-a, B and C) Representative western blot analysis of p-STAT1(Y701) or p-STAT1(S727) in RAW 264.7 cells treated with indicated cytokines for 5 min or specified periods of time. (D) Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h. (E and F-a) Representative western blot analysis of p-STAT1(Y701) or NOS2 in RAW 264.7 cells pretreated with Flu for 24 h prior to cytokine exposure for 5 min or 24 h. (G) Representative western blot analysis of NOS2 in RAW 264.7 cells pretreated with Janus kinase (JAK) inhibitor AG-490 for 30 min prior to cytokine exposure for 24 h. β-actin was used as a loading control. (A-b and F-b) Average quantification obtained by densitometric analysis for western blot analysis. Data are expressed as the density ratio of the target protein to its non-treated level in arbitrary units. Data are presented as the means ± SD from three independent experiments. *p<0.05 and **p<0.01.