Figure 4. let-7g suppresses liver regeneration through insulin-PI3K-mTOR.
(A) Western blots of insulin receptor β, Igf1rβ, Irs2, and β-Actin in negative control or let-7g microRNA mimic treated liver tissues 40 hr after PHx. (B) Quantification of intensity of insulin receptor β, Igf1rβ, Irs2 (Image J). (C) Cell cycle gene expression in let-7S21L alone (n=4) and LAP-let-7S21L (n=4) livers before and 40 hr after PHx as determined by RT-qPCR. (D) Western blots of insulin receptor β, Igf1rβ, p-S6K, total S6K, β-Actin, p-S6 (Ser235/236), and total S6 in AAV-Cre treated let-7b/c2 +/+ and let-7b/c2Fl/Fl livers (n=5 and 5). (E) Quantification of intensity of insulin receptor β/β-Actin, p-S6K/total S6K, and p-S6/total S6, 40 hr after PHx (Image J). (F) Rapamycin treatment during and after PHx in let-7b/c2Fl/Fl control and let-7b/c2 LKO mice. Shown are liver weights 40 hr post PHx. (G) INK128 treatment during and after PHx in let-7b/c2Fl/Fl control and let-7b/c2 LKO mice. Shown are liver weights 40 hr post PHx. (H) Western blots of p-S6K, total S6K, and β-Actin in let-7b/c2Fl/Fl control and let-7b/c2 LKO livers treated with either vehicle or INK128 at 40 hr post PHx. All data in this figure are represented as mean ± SEM. *p<0.05, **p<0.01.