Table 1.
Data collection and refinement statistics.
| Data Set | Dna2-5’ overhang DNA* | Dna2-ssDNA | apo Dna2 | apo Dna2 (SeMet) | DNA2 α1-RPA 70N |
|---|---|---|---|---|---|
| Space group | P22121 | P212121 | P212121 | P212121 | C121 |
| a, b, c (Å) | 87.2, 118.5, 149.3 | 120.2, 149.2, 172.9 | 120.9, 148.6, 170.5 | 120.9, 148.6, 170.5 | 134.3, 50.9, 76.5 |
| α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 103.9, 90.0 |
| Resolution (Å) | 50.0 - 2.35 (2.43 - 2.35) | 30.0 – 3.11 (3.23 - 3.11) | 50.0 – 3.0 (3.11 - 3.0) | 30.0 – 3.4 (3.52 - 3.4) | 60.0 – 1.5 (1.55 - 1.5) |
| Rsym | 12.6 (65.6) |
13.7 (87.5) |
13.1 (78.7) |
15.8 (59.3) |
7.4 (80.1) |
| Rpim | 6.3 (36.6) |
6.6 (57.1) |
6.8 (41.9) |
4.0 (15.1) |
5.1 (54.1) |
| I/σ(I) | 16.4 (1.9) |
13.2 (1.4) |
7.9 (1.3) |
19.3 (4.7) |
23.5 (3.2) |
| Completeness (%) | 99.0 (98.6) |
99.0 (99.2) |
98.3 (98.9) |
100.0 (100) |
84.3 (79.7) |
| Redundancy | 5.0 (3.9) |
6.1 (6.0) |
4.4 (4.5) |
15.1 (15.3) |
2.7 (2.7) |
| Refinement | |||||
| Resolution (Å) | 50.0-2.36 | 30.0–3.15 | 50.0–3.0 | 30.0-1.55 | |
| No. of reflections | 57,871 | 51,122 | 54,107 | 57,455 | |
| Rwork/Rfree (%) | 20.8/24.6 | 22.3/25.6 | 21.1/24.5 | 23.2/26.7 | |
| Protein atoms | 8,298 | 16,536 | 16,536 | 3,933 | |
| DNA atoms | 290 | 674 | 0 | 0 | |
| Cofactor atoms | 41 | 70 | 70 | 0 | |
| Rmsd bond lengths (Å) | 0.009 | 0.009 | 0.009 | 0.007 | |
| Rmsd bond angles (°) B factors (Å2): protein DNA Ca2+ water Wilson |
1.4 65.5 107.6 58.9 49.5 58.4 |
1.4 |
1.46 |
1.3 |
*Only the ssDNA is ordered. Values in parentheses are for the highest-resolution shell.