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Effect of PCDNA3-as-VEGF165 on VEGF mRNA ex-pression of hepatocarcinoma SMMC-7721 cells by RT-PCR. VEGF mRNA expression was negative after PCDNA3-as-VEGF165/LF2000 transfected. (Lane 1: PCDNA3/LF2000 transfected, Lane 2: PCDNA3-as-VEGF165/LF2000 transfected; β-actin: 587 bp, VEGF 258 bp).
