Fig. 1.

SHBG is not expressed in granulosa cells. a RT-PCR for multiple segments of the sex hormone-binding globulin (SHBG) mRNA in human granulosa cells, liver, and testis. Results show no expression of any part of the SHBG mRNA in granulosa cells. The top panel shows SHBG exons 1–6, second panel shows SHBG exons 6–8, and the third panel shows SHBG exons 3–8 with the human testis and liver but not with two pools of GC. The bottom panel shows human GAPDH as positive control. hTestis human testis, hLiver human liver, GC granulosa cells, NTC no template control. b Real-time PCR for multiple segments of SHBG mRNA in the human granulosa cells, liver, and testis. Results show no expression of any part of the SHBG mRNA in granulosa cells. c Western blot analysis for the SHBG protein in human granulosa cells as well as ovarian carcinoma cell line and follicular fluid and actin as a positive control. Results show a clear detection of SHBG in follicular fluid but none in granulosa cells and ovarian carcinoma cell line