Fig. 5.

The expression levels of the five selected lncRNAs (XLOC_011402, ENST00000454271, ENST00000433673, ENST00000450294, and ENST00000432431) were examined by qRT-PCR in a cohort of CC samples isolated from an additional 30 PCOS and 30 normal patients. The expression levels of the five lncRNAs were up-regulated in the cumulus cells of PCOS patients compared to those of normal patients (p < 0.01). The five different lncRNAs are shown on the x-axis. The signal intensity for each lncRNA is shown on the y-axis in arbitrary units determined by qRT-PCR analysis with GAPDH as an endogenous reference. The gray bars show the relative lncRNA expression measured in cumulus cells of PCOS patients. The white bars show the relative lncRNA expression measured in the cumulus cells of normal patients. The results are presented as the means ± SEM