Table 1.
Adiponectin system, Gn-Rs, and r18s primer sequences used to amplify human adiponectin, AdipoR1, AdipoR2, FSHR, and LHR mRNA with RT-PCR and real-time PCR
| Gene | Forward primer (5′–3′) | Reverse primer (3′–5′) | Accession number | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|---|
| Adipo | GCCTACCACATCACAGTC | TCAGCATAGAGTCCATTACG | 001177800 | 46.6 | 200 |
| AdipoR1 | CAGGAAGAAGAGGAGGAG | TATGAATGCGGAAGATGC | 015999 | 53.8 | 226 |
| AdipoR2 | TGGTATTGCTCTTCTGATTATGG | AACACTCCTGCTCTTACTCC | 024551 | 45.8 | 183 |
| FSHR | GAGAGCAAGGTGACAGAGATTC | TTGATGTAGAGCAGGTTGTTGG | 000145 | 56.8 | 236 |
| LHR | CCGGTCTCACTCGACTATCA | ATGCTCCGGGCTCAATGTAT | 000069 | 52.3 | 210 |
| r18s | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | X03205 | 53.5 | 152 |
Adiponectin system: adiponectin, AdipoR1, and AdipoR2; Gn-Rs: FSHR and LHR