Table 1.

Primers used for PCR and subsequent cloning of the genes into the expression vector pET15b

Name	Sequence (5′→3′)	Restriction site
DRF06 FW	GGGAGTACCATATGAGTGATGATGGCCAAC	NdeI
DRF06 RV	ATCCGCTCGAGATAATCTCAAGCCCGCCTCG	XhoI
DRF13 FW	GGGAGTACATATGGATGGCGTGTCAGAC	NdeI
DRF13 RV	CCGCTCGAGTAATGCTTAGGGCCACTTGGC	XhoI
Br FW	CGGACATCATATGTGCCCGCCAG	NdeI
Br RV	ATCCAGGATCCATCGCGTGAACTTCACCACC	BamHI
Rp FW	CGGGACTTCCATATGTCAGGAGCTGAGTTG	NdeI
Rp RV	ACCGCTCGAGTAACGTTCAGCGGAACGC	XhoI
Rr FW	GGGAAATTCCATATGCACCGCCCGGAC	NdeI
Rr RV	GCTCGAGTTCAATTAGCCCTTAACCGCCGC	XhoI

The respective restriction sites are underlined