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Primers for site-directed mutagenesis
| Amino acid exchange | Sequence (5′→3′) | |
|---|---|---|
| R. gelatinosus | H239A | AGCGGCGGACGCGCTCGCTG |
| W241A | CATCGCGCGGGGCCGATCG | |
| H264A | CTGGAGCGGCGCCGCCTACC | |
| Y266A | GCCACGCCGCCCTCGACT | |
| D268A | CGCCTACCTCGCCTCGAACGAAG | |
| T. roseopersicina | H237A | GATCCGGCGGAACGCGCAGTCTGGTGG |
| W239A | CGCCATGTCGCGTGGCCGATC | |
| H262A | GCTGGAGCGGCGCTGGCTAT | |
| D266A | CATGGCTATCTCGCCTCAAA | |
| S58V | GCGTCCGTCGTCGCGCAGCA | |
| S58Q | GCGTCCCAGGTCGCGCAGCA |
Mismatch points are underlined
