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. 2016 Jan 19;6:1552. doi: 10.3389/fmicb.2015.01552

Figure 3.

Figure 3

Endocytosis in Env-expressing cells is required for the cell-cell fusion. (A) The E-MLV R-Env (left panel) or HIV-1 Env (right panel) was transfected to HEK293T cells together with the nls-LacZ expression plasmid. The transfected HEK293T cells were co-culture with TE671mCAT1 or NP2/CD4/X4 cells pretreated with indicated inhibitor (black bars). The transfected cells were treated with indicated inhibitors, and were co-cultured with TE671mCAT1 or NP2/CD4/X4 cells (gray bar). This experiment was repeated three times. Fusion indexes in the control cells were always set to 1, and relative fusion indexes ± SD are shown. Asterisks indicate statistically significant differences between treatments of the Env-expressing or target cells. (B) HEK293T, TE671mCAT1, SC-1, and HeLamCAT1 cells were treated with indicated inhibitor. Ratios (%) of live cells per total cells are indicated. This experiment was repeated three times. (C) HEK293T cells were transfected with the MLV R-Env (left panel) or HIV-1 Env (right panel) expression plsmid together with the nls-LacZ expression plasmid. The cells were also simultaneously transfected with the dynamin dominant negative mutant expression plasmid or pcDNA3.1. This experiment was repeated three times. Fusion indexes in the control cells were always set to 1, and relative fusion indexes ± SD are shown. Asterisks indicate statistically significant differences between transfection with pcDNA3.1 and dynamin mutant.