Table 1.
Screening of proteins present exclusively in tumour supernatant by liquid chromatography tandem mass spectrometry
| Description of protein | Peptide sequence | Mass/Score |
|---|---|---|
| Nuclear migration protein nudC | K.SMGLPTSDEQKK.Q | 885·1/23 |
| Dedicator of cytokinesis protein 7 | R.SYTEDWAIVIRK.Y | 885/19 |
| SET‐binding protein (SEB) | R.SYEGFGTYREK.D | 884·9/23 |
| Dual specificity tyrosine‐phosphorylation‐regulated kinase 4 | R.NLKPQPRPQTLRK.S | 860·8/18 |
| Toll‐like receptor 6 precursor (CD286 antigen) | K.SIVENIINCIEK.S | 860·4/15 |
| Microtubule‐associated proteins 1A/1B light chain 3C precursor (Microtubule‐associated protein 1 light chain 3γ) (MAP1A/MAP1B LC3 C) (MAP1A/1B light chain 3 C) (MAP1 light chain 3‐like protein 3) (Autophagy‐related protein LC3 C). | K.SLVSMSATMAEIYR.D | 860·4/17 |
| U6 snRNA‐associated Sm‐like protein LSm2 | K.SLVGKDVVVELK.N | 860·1/19 |
| SWI/SNF‐related matrix‐associated actin‐dependent regulator of chromatin subfamily A containing DEAD/H box 1 (EC 3·6·1.‐) | K.LNYAIFDEGHMLK.N | 855·4/15 |
| Protein GPR89A (Putative MAPK‐activating protein PM01) | R.RLLQTMDMIISK.K | 590·6/20 |
| Ephexin‐1 (Eph‐interacting exchange protein) | K.SVNEPLTLNIPWSR.M | 590·3/15 |
| Semenogelin‐1 precursor | K.EQTSVSGAQK.G | 590·3/17 |
| Aryl hydrocarbon receptor nuclear translocator 2 (ARNT protein 2) | R.SGMDFDDEDGEGPSK.F | 582·3/22 |
| Pleckstrin homology‐like domain family B member 2 | R.SGAASMPSSPK.Q | 582/23 |
| Kinesin‐like protein KIF14 | R.SGHLTTKPTQSK.L | 534·0/16 |
| Chromatin‐modifying protein 4c | K.KHGTQNK.R | 478·3/16 |
| PHD finger protein 21B | R.HQNGDLK.K | 478·3/16 |
| CD109 antigen precursor (p180) | R.TYTWLK.G | 478·3/16 |
| Ankyrin repeat and SOCS box protein 1 (ASB‐1) | K.WESLGPESRGR.R | 473·3/14 |
| Interleukin‐1 family member 8 (IL‐1F8) | R.TNIGMPGR.M | 502·8/11 |
| Mastermind‐like protein 3 (Mam‐3) | R.SLQGMPGR.T | 502·8/11 |
| Kinesin‐like protein KIF14 | R.SGHLTTKPTQSK.L | 524·9/27 |
| Microtubule‐associated proteins 1A/1B light chain 3C precursor | K.SLVSMSATMAEIYR.D | 860·4/15 |
| Integrin α‐L precursor | R.RGLFPGGR.H | 503·8/14 |
| Ras‐related protein Rab‐6B | M.SAGGDFGNPLRK.F | 503·3/14 |
| Kelch domain‐containing protein 5 | R.SNFKLVAVNSK.L | 450·8/11 |
| 2′‐5′‐oligoadenylate synthetase 2 (EC 2·7·7.‐) | R.ILNNNSK.R + | 473·3/23 |
| Epididymal secretory protein E1 precursor | K.TYSYLNK.L | 517·3/19 |
| Pericentrin (Pericentrin B) (Kendrin) | R.SLTEQQGR.L | 531·2/22 |
| Ubiquitin‐conjugating enzyme E2 J1 (EC 6·3·2·19) | R.GPPDSDFDGGVYHGR.I | 573·8/16 |
| Protocadherin β16 precursor (PCDH‐β16) | R.VIDINDHSPMFTEKEMILK. | 855·1/23 |