Fig. 3.

Analysis of CpG dinucleotide methylation in the Oct4 gene promoter and expression of genes specific for pluripotent cells in common vole iPSCs. A – comparison of CpG dinucleotide methylation of the Oct4 promoter in the obtained iPSC lines (14vf2, 14vf7) and initial line (vEFSkRA) of embryonic skin fibroblasts. Schematic distribution of CpG dinucleotides in a mouse and a common vole Oct4 promoter is presented at the top. Light and dark circles – unmethylated and methylated CpG dinucleotides, respectively. B – transcriptional activity of the genes responsible for the pluripotent state in the iPSC lines 14vf2 and 14vf7 and their differentiated derivatives (14vf2-EB and 14vf7-EB). Control – initial cell line vEFSkRA. (RT-) – negative control of reverse transcription reaction