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Verification of deletions/insertions by PCR. PCR products formed from DNA of the parent clone 14 VACV LIVP and 1421ABJCN with disrupted virulence genes (see text for details). M – molecular size marker, the lengths are given on the left . Lanes A, B, C, N, J contain VACV DNA fragments amplified from the genes A56R, B8R, C3L, N1L, and J2R, respectively
