Fig. 1.

Electrophoretic analysis of the studied BChE-based biopharmaceuticals and their conjugates with polysialic acids (CAO). Separation was carried out in 8% PAGE under non-reducing conditions, followed by Coomassie R-250 staining. An rhBChE sample is present as a mixture of monomeric, dimeric, and tetrameric forms; a 4rhBChE sample is present exclusively in the tetrameric form. Polysialylated BChE samples obviously have higher molecular weights, but broad bands; this effect was described for chemically polysialylated proteins [17]