Table 3.
Predicted Laccaria metabolism correlates with clustered aspen root gene expression.
| Correlates with Aspen Gene Cluster | Laccaria Metabolite | Relevant KEGG Pathway |
|---|---|---|
| Cluster 1 | Caffeoyl-CoA | Biosynthesis of phenylpropanoids |
| Salicylate | Biosynthesis of plant hormones | |
| Pyruvate | ||
| trans-2,3-Dihydroxycinnamate | Degradation of aromatic compounds | |
| 3-(2,3-Dihydroxyphenyl) propanoate | ||
| Hydantoin-5-propionate | Histidine metabolism | |
| Dolichyl beta-D-glucosyle phosphate | N-Glycan biosynthesis | |
| Dolichyl diphosphate | ||
| N-Hydroxyphenylacetate | Phenylalanine metabolism | |
| Dextrin | Starch and sucrose metabolism | |
| N-Hydroxyl-tryptamine | ||
| 2-Octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone | Ubiquinone and other terpenoid-quinone biosynthesis | |
| Aldoxime | ||
| 4-Hydroxymandelonitile | ||
| Cluster 2 | Pyruvate | Biosynthesis of plant hormones |
| 1-Phosphatidyl-D-myo-inositol | N-Glycan biosyntesis | |
| Dolichyl beta-D-glucosyle phosphate | ||
| Dextrin | Starch and sucrose biosynthesis | |
| Cluster 5 | L-Gilonon-1,4-lactone | Ascorabate and aldarate metabolism |
| beta-D-Fructose 1,4-bisphosphate | Biosynthesis of plant secondary metabolites | |
| Pyruvate | ||
| Ocanoyl-CoA | ||
| Lauroyl-CoA | ||
| Tetradecanol-CoA | Fatty acid metabolism | |
| Decanoyl-CoA | ||
| Hexanoyl-CoA | ||
| Cluster 6 | Benzoate | Biosynthesis of alkaloids derived from shikimate pathway |
| p-Coumaroyl-CoA | Biosynthesis of phenylpropanoids | |
| L-Asparagine | Biosynthesis of plant secondary metabolites | |
| Tryptamine | ||
| (Z)-4-Hydroxyphenylacetaldehyde-oxime | Glucosinolate biosynthesis | |
| 4-Imidazolone-5-propanoate | Histidine metaolism | |
| Nitrile | Nitrogen metabolism | |
| Fe2+ | ||
| 3-Coumaric acid | Phenylalanine metabolism | |
| 3-(3-Hydroxyphenyl)-propanoic acid | Ubiquinone and other terpenoid-quinone biosynthesis | |
| 2-Hexaprenyl-6-methoxyphenol | ||
| 2-Ocatprenyl-3-methyl-6-methoxy-1,4-benzoquinone |
To identify potential signaling molecules and classes of signaling molecules synthesized by Laccaria and detected by aspen root, predicted differentially metabolized Laccaria molecules were correlated with observed patterns of aspen root gene expression. Column “Aspen Gene Cluster” identifies the aspen co-expressed gene clusters from Figure 4. “Laccaria Metabolite” lists the predicted Laccaria metabolites with PRMT-scores that strongly, negatively correlate (0.005/0.5th percentile of all correlations between gene expression patterns and PRMT-scores) with aspen root gene co-expression patterns. While a metabolite might belong to multiple KEGG pathways (Ogata et al., 1999), the KEGG pathway predicted to be most relevant to Laccaria-aspen signaling compounds is identified for each metabolite. Specific KEGG pathways likely to be relevant to mycorrhizal signaling are highlighted in bold.