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. 2016 Jan 19;6:1267. doi: 10.3389/fpls.2015.01267

FIGURE 1.

FIGURE 1

Hematin (Ht), sodium nitroprusside (SNP), zinc protoporphyrin IX (ZnPPIX), and 2-(4-carboxyphenyl0-4, 4, 5, 5-tetramethylimidazoline-l-oxyl-3-oxide potassium salt (cPTIO) up- or down-regulated HO-1 gene expression (A) and HO activity (B) in rice aleurone layers subjected to drought stress. Rice aleurone layers were treated with or without 20% (polyethylene glycol-6000) PEG, 1 μM Ht, 200 μM SNP, 10 μM ZnPPIX, 200 μM cPTIO individually or in combination for 24 h. A sample with distilled water was used as control (C). Measurement of HO-1 transcript levels in the rice aleurone layers was conducted by real-time fluorescence quantitative PCR assay (A), HO activities were also detected (B). Mean values were calculated from at least three independent experiments, bars with different letters indicate statistically significant differences at the 0.05 level, using Duncan’s multiple test.