Figure 3.

Schematic overview of the daily time-place learning (TPL) testing protocol for two time points and two daily sessions (2-arm TPL design) or three time points and three daily sessions (3-arm TPL design). Mice had to learn to avoid one non-target location, which changed depending on the time of day (TOD; i.e., session). Open circles indicate food (powdered standard rodent chow, <0.1 g) at the end of an arm of the maze (see Figure 5A); gray circles indicate the (non-target) shock location. Mice were tested individually two or three times a day (depending on the design) in 10 min trials, with an intersession time of 3 h. Bodyweights were taken before each trial. Mice received an individual amount of food at the end of each day in order to maintain body weight at 85–87% of ad libitum feeding weight. Testing was performed in the light phase. ZT0 (zeitgeber time zero) indicates lights on.