FIGURE 1.

Workflow of the nanoString strategy. Capture probes are bound to biotin and reporter probes consist of four different fluorophores that are arranged at six different positions, thus providing a specific fluorescent signature for each probe. After mRNA hybridization and removal of excess unbound material, hybrid molecules are fixed onto a surface using electrical current which will orient all molecules. Sequential reading with a fluorescent source provides signal signatures for each specific mRNA in a quantitative manner. Adapted from Fortina and Surrey (2008).