FIGURE 2.

Workflow of the mRNA enrichment strategy using the SureSelect technology. The technology consists of capturing the mRNAs of interest out of the population of total RNA molecules transcribed from mixed genomes. A library of biotinylated oligonucleotides (corresponding to an ORFome and consisting of overlapping oligonucleotides for each gene) is hybridized in a solution with a sample (cDNA from total RNA). Biotylinated RNA-cDNA hybrids can be selectively captured with magnetic streptavidin-coated beads. After digestion of the biotinylated RNA baits, the remaining cDNA can be sequenced by high throughput sequencing approaches (RNA-seq).