Figure 3.

Limited cross-reactivity between HCV genotype-3 and genotype-1 in spontaneously resolved infection. HCV genotype-3 and genotype-1 specific T cell responses were measured by IFNγ-ELISpot assays (spot-forming units (SFUs)/10⁶ peripheral blood mononuclear cells (PBMC)) using (A) an HCV genotype-3 and genotype-1 specific overlapping peptide set spanning the entire HCV genome; or (B) HCV genotype-3 and genotype-1 individual peptide variants at T cell targets detected in spontaneously resolved infection. Sequence variants identical between HCV genotype-1a and 1b are marked by a bar, those not assessed by IFNγ-ELISpot assays are marked with a star. (C) Significantly reduced T cell cross-reactivity at T cell targets identified in spontaneously resolved infection was detected against common HCV genotype-1a and genotype-1b sequence variants at individual peptide level.