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. 2015 Sep 8;69(1):35–41. doi: 10.1136/jclinpath-2015-203097

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pTEN protein expression. (A) Negative, (B) grade 1—weak cytoplasmic staining, less intense than the surrounding stroma, (C) grade 2—moderate cytoplasmic staining, where staining is equal in intensity to the adjacent stromal staining and (D) grade 3—strong cytoplasmic staining, where staining is stronger in intensity to the adjacent stromal staining (×200 magnification).