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Constructed pET-ret plasmid after cloning of reteplase gene in pET-21; A) Purification from E. coli DH5α. M: molecular weight marker, 1:3 μl of plasmid on 1% agarose gel. B) PCR amplification of reteplase gene (1065 bp) using pET-ret as the template, M: molecular weight marker, 1:5 μl of the PCR product on 1% agarose gel.
