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. 2016 Jan-Mar;8(1):16–22.

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Copyright© 2016 Avicenna Research Institute

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Figure 4. — Analysis of recombinant reteplase expressed in E. coli SHuffle T7 at 37°C and 1 mM IPTG. A) The insoluble and B) soluble fractions of cell lysates were separated and loaded on 10% SDS-PAGE. M: prestained molecular weight marker, fractions were withdrawn before induction of expression (lane 1), at 1 hr (lane 2), 2 hr (lane 3), 4 hr (lane 4) and 20 hr (lane 5) after induction.