Table 1.
Primer sequences and conditions used in qRT-PCR analysis
| Gene | Sequences (forward and reverse) | Tm(°C) | Product size (bp) |
|---|---|---|---|
| OCT4 | 5′-TTC GCA AGC CCT CAT TTC AC-3′ 5′-CCA TCA CCT CCA CCA CCT G-3′ |
60 | 114 |
| FOXA2 | 5′-AGC GAG TTA AAG TAT GCT GG-3′ 5′-GTA GCT GCT CCA GTC GGA-3′ |
58 | 93 |
| SOX17 | 5′-CAA GAT GCT GGG CAA GTC-3′ 5′-TGG TCC TGC ATG TGC TG-3′ |
60 | 93 |
| CXCR4 | 5′-CGC CAC CAA CAG TCA GAG-3′ 5′-AAC ACA ACC ACC CAC AAG TC-3′ |
58 | 176 |
| Beta2M | 5′-ATG CCT GCC GTG TGA AC-3′ 5′-ATC TTC AAA CCT CCA TGA TG-3′ |
56 | 91 |