Fig. 1.

Immunohistochemical detection of P. gingivalis in normal esophageal mucosa, and cancerous and adjacent tissues of ESCC. a, b, and c are representative images of P. gingivalis in well differentiated (a), moderately differentiated (b), and poorly differentiated (c) ESCC tissues. Pre-immune rabbit IgG was used as a control to detect the serial tissue sections from the same paraffin-embedded tissue block of: (d) well differentiated-; (e) moderately differentiated-; and (f) poor differentiated ESCC. g Normal esophageal mucosa stained with anti-P. gingivalis anti-serum; (h) and (i) are the representative negative/positive images of P. gingivalis immunostaining in the adjacent cancerous tissues. 20× magnification; scale bar = 50 μm; miniatures in the left corners amplify the area with red arrows