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. 2016 Jan 19;16:28. doi: 10.1186/s12885-016-2061-3

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© Samulin Erdem et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — a Efficient SOX2 and TP53 knockdown was confirmed by western blotting. SOX2 and TP53 were silenced using siRNA technology. After 48 h nuclear lysates were isolated from knockdown and control cells. 40 µg lysate/lane was resolved in a SDS-PAGE and membrane analysed for expression of SOX2, TP53 and GAPDH (loading control) by western blotting. b Protein expression was semiquantitatively analysed by densitometry using Image J. Bars indicate mean ± SE, n = 3. ***indicating p < 0.001 and **indicating p < 0.01 was obtained from one-way ANOVA