Table 1.
Amplified factor | primers 5′ to 3′ | Product size (bp) | AT (°C) | Genbank accession number | |
---|---|---|---|---|---|
miR-21 | S | TGTCGGGTAGCTTATCAGAC | ~90 | 55 | NR_029493 |
AS | Adapter primer in kit | ||||
U6-snRNA | S | GCTTCGGCAGCACATATAC | ~120 | 55 | NR_004394 |
AS | Adapter primer in kit | ||||
PTEN | S | ACCAGAGACAAAAAGGGAGTA | 173 | 56 | NM_000314 |
AS | ACCACAAACTGAGGATTGCA | ||||
HPRT | S | TGGACTAATTATGGACAGGACT | 219 | 56 | NM_000194 |
AS | CCTGTTGACTGGTCATTACAAT | ||||
PTEN Promoter | |||||
Unmethylated (−300)a | S-U | TGGGTTTTGGAGGTTGTTGGT | 173 | 55 | NG_007466.2 |
AS-U | ACTTAACTCTAAACCACAACCA | ||||
Methylated (−298)a | S-M | GGTTTCGGAGGTCGTCGGC | 155 | 57 | |
AS-M | CAACCGAATAATAACTACTACGACG |
S sense, AS antisense, U unmethylated, M methylated, AT annealing temperature
aDistance of the 5′ nucleotide of the sense primer from the transcription start site of PTEN gene