Table 5.
Diet | Week | adequate-Se | Low-Se | p-value | ||||
---|---|---|---|---|---|---|---|---|
n | mean | SE | n | mean | SE | |||
GPx1 | 0 | 12 | 5.52 | 0.90 | 12 | 6.29 | 1.17 | 0.611 |
2 | 12 | 5.93a | 1.15 | 12 | 6.03a | 1.35 | 0.956 | |
4 | 12 | 4.80a | 0.77 | 12 | 4.62a | 0.59 | 1.000 | |
6 | 12 | 4.42a | 0.49 | 12 | 5.92a | 1.24 | 0.950 | |
8 | 12 | 5.30a | 0.48 | 12 | 6.27a | 1.28 | 0.992 | |
SelH | 0 | 12 | 2.68 | 0.19 | 12 | 3.12 | 0.23 | 0.154 |
2 | 12 | 3.58a | 0.36 | 12 | 4.08a | 0.26 | 0.972 | |
4 | 12 | 3.05a | 0.29 | 12 | 3.38a | 0.46 | 0.993 | |
6 | 12 | 2.20b | 0.27 | 12 | 2.37b | 0.19 | 1.000 | |
8 | 12 | 2.27b | 0.36 | 12 | 2.20b | 0.18 | 0.954 | |
SepW1 | 0 | 12 | 2.16 | 0.24 | 12 | 2.62 | 0.35 | 0.286 |
2 | 12 | 2.41a | 0.31 | 12 | 2.41a | 0.26 | 0.870 | |
4 | 12 | 2.48a | 0.41 | 12 | 2.41a | 0.44 | 0.581 | |
6 | 12 | 2.07a | 0.26 | 12 | 2.05a | 0.20 | 0.868 | |
8 | 12 | 2.61a | 0.72 | 12 | 2.03a | 0.30 | 0.468 | |
TrxRd1 | 0 | 12 | 2.00 | 0.09 | 12 | 1.96 | 0.06 | 0.721 |
2 | 12 | 5.54a | 0.23 | 12 | 4.77a | 0.38 | 0.259 | |
4 | 12 | 3.97b | 0.26 | 12 | 3.89a | 0.37 | 0.995 | |
6 | 12 | 1.89bc | 0.15 | 12 | 1.67b | 0.11 | 0.681 | |
8 | 12 | 1.64bc | 0.09 | 12 | 1.65b | 0.15 | 1.000 | |
TrxRd2 | 0 | 12 | 2.86 | 0.19 | 12 | 3.01 | 0.20 | 0.615 |
2 | 12 | 4.04a | 0.40 | 12 | 3.84a | 0.21 | 1.000 | |
4 | 12 | 3.75a | 0.40 | 12 | 3.61ac | 0.42 | 0.993 | |
6 | 12 | 2.25b | 0.27 | 12 | 2.48bc | 0.24 | 0.910 | |
8 | 12 | 3.17ab | 0.64 | 12 | 2.31b | 0.26 | 0.452 | |
SelK | 0 | 12 | 13.10 | 0.78 | 12 | 13.72 | 0.77 | 0.575 |
2 | 12 | 2.78a | 0.25 | 12 | 2.95a | 0.23 | 0.998 | |
4 | 12 | 2.68a | 0.25 | 12 | 2.80a | 0.33 | 1.000 | |
6 | 12 | 11.42b | 0.58 | 12 | 12.89b | 1.17 | 0.982 | |
8 | 12 | 17.96c | 2.01 | 12 | 15.91b | 1.61 | 0.538 | |
SelT | 0 | 12 | 9.72 | 1.17 | 12 | 10.25 | 1.11 | 0.746 |
2 | 12 | 4.27a | 0.52 | 12 | 4.72a | 0.52 | 0.921 | |
4 | 12 | 4.85a | 0.63 | 12 | 5.05a | 0.67 | 0.998 | |
6 | 12 | 7.12b | 0.74 | 12 | 7.97b | 1.07 | 1.000 | |
8 | 12 | 7.57b | 0.86 | 12 | 7.92b | 0.85 | 1.000 | |
Sep15 | 0 | 12 | 2.37 | 0.15 | 12 | 2.47 | 0.19 | 0.693 |
2 | 12 | 1.90ac | 0.14 | 12 | 1.97a | 0.11 | 0.996 | |
4 | 12 | 1.87a | 0.20 | 12 | 1.71a | 0.15 | 0.788 | |
6 | 12 | 2.44b | 0.16 | 12 | 2.66b | 0.21 | 0.962 | |
8 | 12 | 2.42bc | 0.25 | 12 | 2.67b | 0.31 | 0.953 | |
TNF-α | 0 | 12 | 3.28 | 0.30 | 12 | 3.54 | 0.38 | 0.597 |
2 | 12 | 6.73a | 0.91 | 12 | 7.03a | 0.67 | 0.990 | |
4 | 12 | 6.73a | 0.54 | 12 | 6.95a | 0.66 | 1.000 | |
6 | 12 | 2.89b | 0.40 | 12 | 3.31b | 0.51 | 0.909 | |
8 | 12 | 3.29b | 0.34 | 12 | 2.91b | 0.29 | 0.681 | |
NFκB | 0 | 12 | 2.07 | 0.11 | 12 | 2.17 | 0.12 | 0.543 |
2 | 12 | 5.18a | 0.35 | 12 | 5.19a | 0.21 | 1.000 | |
4 | 12 | 6.55a | 0.34 | 12 | 6.26a | 0.47 | 0.946 | |
6 | 12 | 1.72b | 0.09 | 12 | 1.68b | 0.13 | 0.982 | |
8 | 12 | 1.87b | 0.16 | 12 | 1.71b | 0.12 | 0.821 |
aSamples with the lowest number of gene copies for each gene of interest had a value of 1 with all other sample values relative to that. Values at weeks zero indicate the average baseline values. Weeks within one gene and diet with a common letter in superscript (mean column) do not significantly differ (p > 0.05). qPCR, quantitative polymerase chain reaction; Se, selenium; n, number of dogs; SE, standard error; GPx1, glutathione peroxidase 1; SelH, selenoprotein H; SepW1, selenoprotein W; TrxRd1, thioredoxin reductase 1; TrxRd2, thioredoxin reductase 2; DIO1, iodothyronine deiodinase 1; SelK, selenoprotein K; SelT, selenoprotein T; Sep15, 15 kDa selenoprotein; TNF-α, tumor necrosis factor alpha; NFκB1, nuclear factor kappa-light-chain-enhancer of activated B cells; B2M, beta-2-microglobulin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HPRT1, hypoxanthine phosphoribosyltransferase 1