Fig. 3.

The working principle for screening of cell-specific peptides from a phage display library. A phage display library was incubated with the cells. Unbound phage was removed using washing buffer, and cells bound to phage were eluded and amplified with E. coli in broth. Enriched phages were used in the next round of panning. After three to five rounds of selection panning, the positively selected phages were collected and sequenced