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. Author manuscript; available in PMC: 2016 Jan 19.
Published in final edited form as: Nat Struct Mol Biol. 2014 Nov 10;21(12):1091–1096. doi: 10.1038/nsmb.2905

Figure 3.

Figure 3

Ligands of αVβ6. (a) RGD sequences from pro-TGF-β and VP1 protein from FMDV. (b) Competitive binding affinities of TGF-β3 peptide truncations. Fluorescence anisotropy data are mean ± s.e.m. of technical triplicate samples scaled logarithmically. (c) Western blot of pro-TGF-β1 secreted by the indicated HEK293T transfectants, with antibody to the prodomain as described previously11. (d) TGF-β bioassay of pro-TGF-β1 and its double-proline mutant. Data show mean ± s.e.m. of technical triplicate samples.