Fig. 7.

Effects of U0126, apocynin, or p47phox knockout on microglial differentiation 1 day after MA. Microglial differentiation into M1 type (a–c) and into M2 type (d–e). Gene primer sequences for RT-PCR analysis were shown in Table 1. WT wild-type mice, p47 KO p47phox knockout mice, Sal saline, U U0126 (2 μg, i.c.v.), Apo apocynin (50 mg/kg, i.p.), V or Veh vehicle [10 % (v/v) DMSO] for U0126 or apocynin. Each value is the mean ± S.E.M. of six animals. ^** P < 0.01 vs. vehicle/WT with saline. ^# P < 0.05 vs. vehicle/WT with MA (three-way ANOVA was followed by Fisher’s LSD pairwise comparisons)