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Schematic illustrating the Stable Isotope Standards and Capture by Anti-Peptide Antibodies (SISCAPA) approach. The targeted peptides from the cytokines are listed in black. The peptide sequence denoted in red (GSGC) is added to allow for a more immunogenic response during antibody production. Polyclonal antibodies are retrieved and immobilized for affinity chromatography capture followed by RPLC-separation and MS/MS detection.
