Figure 1.

Evaluation of the cytotoxicity of apigenin (API) on BMDCs. (A and B) BMDCs were generated from bone marrow of C57BL/6 mice and purified with anti‐CD11c‐coated magnetic beads using the autoMACS system. Purified BMDCs (>95% for CD11c⁺ cells) were treated with various concentrations of API in the absence or presence of LPS for 24 hrs, and then stained with Annexin V‐FITC/Propidium Iodine Apoptosis Detection Kit (Invitrogen, Carlsbad, CA, USA). Apoptotic and necrotic rates were determined by flow cytometry. The values are means ± SEM of samples from three wells. **P < 0.01 were for the comparisons between API‐treated and LPS‐ induced DCs versus API 0 μM.