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. 2015 Oct 30;20(1):134–146. doi: 10.1111/jcmm.12712

Figure 3.

Figure 3

Human mesenchymal stromal cells (hMSCs) purified from umbilical cord blood (UCB) express functional SexH receptors. (A) RT‐PCR (n = 2) for SexH (pituitary and gonadal) receptor expression in purified mRNA samples from cultured UCB‐derived MSCs (Lane 1) and human ovarian cancer cell line cells (hOCCs) employed as positive control (Lane 2). Samples with water only instead of cDNA template were used as negative controls (Lane 3). A representative agarose gel of the RT‐PCR amplicons is shown. Expression of these SexH receptors was also confirmed on human MSCs by immunofluorescence staining (Fig. S3) (n = 2). (B) The effect of pituitary and gonadal SexHs on the phosphorylation of p42/44 MAPK and AKT (Ser473) in human UCB‐derived MSCs, which were starved for 16 hrs in DMEM medium containing 0.5% BSA in an incubator and afterwards stimulated by SexHs (FSH [10 IU/ml], LH [10 IU/ml], PRL [2 μg/ml], estradiol [0.1 μM] or danazol [4 mg/ml]) for 5 min. before collecting protein lysates. One set of representative blots out of two is shown. (C) Densitometric analysis of blots shown in (B). The experiment was repeated twice on isolated cells with similar results, and representative images are shown.