Figure 4.

PMSCs improves pathologies associated with ischemia injury to hindlimbs. (A) At day 21 after treatment, hematoxylin and eosin staining for ischemic limbs showed massive muscle degeneration in the PBS group. In the PMSCs group, the muscle degeneration of limb ischemia was largely prevented. Masson's trichrome staining showed that fibrosis was obviously attenuated in PMSCs‐treated limbs. (B) Muscle fibre numbers per mm² were quantified in order to show the regeneration rate of muscle fibre in different groups. (C) Fibrotic area was calculated based on Masson's trichrome staining. Data were presented by mean ± SD, *P < 0.05.