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. Author manuscript; available in PMC: 2016 Jan 19.
Published in final edited form as: Gastroenterology. 2010 Oct 16;140(3):903–912. doi: 10.1053/j.gastro.2010.10.012

Figure 2.

Figure 2

Verification of fluorescence-activated cell sorting (FACS) purification of cholecystokinin (CCK)–enhanced green fluorescent protein (eGFP) cells by gene expression using Taqman reverse transcriptase polymerase chain reaction. Expression of CCK messenger RNA was high relative to β-actin (×103) in CCK-eGFP cells with nearly undetectable contaminating transcripts for enterocyte (alkaline phosphatase [AKP3]) and goblet cell (calcium/chloride channel [CLCA3]) markers compared to a simultaneously sorted non-eGFP population (n = 5–6 separate cell isolations).