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Primer and probe sequences
| Genes | Forward | Reverse |
|---|---|---|
| ID4 BSP primer | GTTTGATTGGTTGGTTATTTTAGAT | ACCGAAAAAAAAATAACCCAC |
| CACCAAAAAAAAAATAACCCAC | ||
| ID4 methylight | ||
| Primer | TCGGAGTTTTCGTTTTCGTT | CGATACTACTCACAACCGCG |
| Probe | AGCGGGTTTCGTTCGGTTCG | |
| MYOD1 methylight | ||
| Primer | CCAACTCCAAATCCCCTCTCTAT | TGATTAATTTAGATTGGGTTTAGAGAAGGA |
| Probe | TCCCTTCCTATTCCTAAATCCAACCTAAATACCTCC |
BSP: Bisulfite sequencing polymerase chain reaction.
