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SDS-PAGE and Western blotting analysis of heat shock protein/peptides of S180 cells after Concanavalin A affinity chromatography. Coomassie light-blue staining of a 10% SDS-PAGE gel. Lane 1, standard; lane 2, fractions 3-6. Western blot analysis: Lane 3, proteins that did not bind the ATP column; lane 4, protein that bound to ATP; lane 5, anti-GRP96 antibody staining; lane 6, anti-heat shock protein 110 antibody staining.
