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. 2015 Nov 30;35(1):62–76. doi: 10.15252/embj.201591973

Figure 2. PLCβ1 and PTPRN2 drive metastatic migration and invasion.

Figure 2

  • A
    Matrigel invasion by 50,000 LM2 cells transfected with siRNA targeting PTPRN2, PLCβ1, or control siRNA. Data normalized to control values. N = 6 inserts/group.
  • B
    Migration assay by 100,000 LM2 cells transfected with siRNA targeting PTPRN2, PLCβ1, or control siRNA. Data normalized to control values. N = 6 inserts/group. Right, representative images of the migration assay. Scale bar, 100 μm.
  • C
    Quantification of area covered by cells 24 h after a scratch was made through confluent cells transfected with siRNA targeting PTPRN2, PLCβ1, or control siRNA. N = 5 wells/group. Right, representative images of the scratch assay.
  • D, E
    MDA‐MB‐231 cells transduced with PTPRN2, PTPRN2C945A, or control vector were subjected to the Matrigel invasion (D) and migration assays (E). N = 5 inserts/group.
  • F
    Bioluminescence imaging quantification of lung colonization by 40,000 MDA‐MB‐231 cells overexpressing PTPRN2, PTPRN2C945A, or control vector. N = 5 mice/group. Right, H&E staining of representative lung sections.
  • G, H
    MDA‐MB‐231 cells transduced with PLCβ1, PLCβ1H331Q, or control vector were subjected to the Matrigel invasion (G) and migration assays (H). N = 5 inserts/group.
  • I
    Bioluminescence imaging quantification of lung colonization by 40,000 MDA‐MB‐231 cells overexpressing PLCβ1, PLCβ1H331Q, or control vector. For Cntrl and PLCβ1H331Q OE: N = 6 mice/group. For PLCβ1 OE: N = 5 mice. Right, H&E staining of representative lung sections.
Data information: Error bars represent SEM. *P < 0.05, **P < 0.01, ***P < 0.001.