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. 2016 Jan 13;18:10. doi: 10.1186/s13075-015-0901-1

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© Lacerte et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Intracellular cytokine expression in monocyte subsets from rheumatoid arthritis (RA) patients following toll-like receptor 9 (TLR9) stimulation. Mononuclear cells from RA patients and healthy donors were enriched and stimulated with CpG-2006 (a) and Epstein–Barr virus (EBV) DNA (TLR9 ligands) (b) in vitro. Gating strategy is described in Fig. 5. Intracellular cytokine level for IL-6, IL-1β, TNFα and monocyte chemoattractant protein-1 (MCP-1) are shown for all three subsets of monocytes. Gray non-stimulated samples, Blue healthy donors, red RA patients. Representative cytometry histograms are representative of ten donors